Steelers C Maurkice Pouncey and DE Aaron Smith did not practice on Friday and have been ruled out for the Super Bowl on Sunday.
Smith was not expected to be able to play, but Pittsburgh was holding out hope for Pouncey, its All-Pro rookie center.
"Obviously he's a quality player, and how it affects the game, no one knows," Steelers head coach Mike Tomlin said. "But as far as what we do, we have a plan, and that won't change.''
Pouncey had said earlier in the week that he likely would not play if he couldn't practice on Friday.
He started every game in his rookie campaign prior to the Super Bowl, and is suffering from a high ankle sprain. OG Doug Legursky, who made four starts in the regular season, will replace Pouncey.
Smith has been out since Oct. 24 with a torn triceps.
The way we see it
This not a surprise, and the Steelers should be well prepared for life without Pouncey since Legursky played most of the AFC championship game victory over the Jets.